Novel effectors of dipeptidyl peptidase IV

ABSTRACT

Dipeptide compounds and compounds analogous to dipeptide compounds that are formed from an amino acid and a thiazolidine or pyrrolidine group, and salts thereof used in the treatment of impaired glucose tolerance, glycosuria, hyperlipidaemia, metabolic acidoses, diabetes mellitus, diabetic neuropathy and nephropathy and also of sequelae of diabetes mellitus in mammals.

CROSS REFERENCE TO OTHER APPLICATIONS

The present application is a continuation of application U.S. Ser. No. 10/361,956, filed on Feb. 10, 2003, which is a continuation of application U.S. Ser. No. 09/723,638, filed on Nov. 28, 2000, which claims priority of DE 198/23831.2 filed on May 28, 1998 and subsequent PCT EP 99/03712 application filed on May 28, 1999.

BACKGROUND OF THE INVENTION

According to the current state of the art, hyperglycaemia and associated causes and sequelae (including diabetes mellitus) are treated by the administration of insulin (e.g. material isolated from bovine pancreas or obtained by genetic engineering techniques) to the diseased organisms in various forms of administration. All methods known hitherto, including more modern procedures, are distinguished by the requirement of a large amount of material, by high costs and often by a distinct impairment of the quality of life of the patients. The conventional method (daily i.v. insulin injection, customary since the 1930s) treats the acute symptoms of the disease, but after prolonged use leads inter alia to serious vascular changes (arteriosclerosis) and nerve damage.

More recently the installation of subcutaneous depot implants (the insulin is released in metered amounts, and daily injections are unnecessary) and implantation (transplantation) of intact Langerhan's cells into the functionally impaired pancreatic gland or into other organs and tissues have been proposed. Such transplants require a high level of technical resources. Furthermore, they involve a surgical intervention into the recipient organism, which is associated with risks, and even in the case of cell transplants require methods of suppressing or circumventing the immune system.

The use of alanyl pyrrolidide and isoleucyl thiazolidide as inhibitors of DP IV or of DP IV-analogous enzyme activity is already known from PCT/DE 97/00820 and the use of isoleucyl pyrrolidide and isoleucyl thiazolidide hydrochloride is already known from DD 296 075. Isoleucyl thiazolidide, which is used in the latter prior art, is a natural, that is to say L-threo-isoleucyl thiazolidide: on the priority date and also on the application date of the two specifications, only that form, the natural form, of isoleucyl thiazolidide was available.

It has been established that those compounds, especially L-threo-isoleucyl thiazolidide, are good effectors for DP IV and DP IV-analogous enzyme activities, but the use of that compound may give rise to certain problems in the case of some patients or some forms of the disease:

Depending upon the symptoms and the severity e.g. of diabetes mellitus it would be desirable, for example, to have available effectors that have an action different from that of the known compounds: for example, it is known that diabetes mellitus patients must be “stabilised” individually in order that their illness can be treated in an optimum manner. In some cases, for example, a reduction in the activity by DP IV effectors ought to be sufficient. It is also possible that too high a level of inhibitor activity and the permanent administration of the same medicament, especially in view of the life-long duration of treatment, may result in undesirable side-effects. Furthermore, it could also be desirable to improve certain transport properties in order to increase the rate of absorption of the effectors in vivo

SUMMARY OF THE INVENTION

The present invention relates to dipeptide compounds and compounds analogous to dipeptide compounds that are formed from an amino acid and a thiazolidine or pyrrolidine group, and salts thereof, referred to hereinafter as dipeptide compounds, and to the use of the compounds in the treatment of impaired glucose tolerance, glycosuria, hyperlipidaemia, metabolic acidoses, diabetes mellitus, diabetic neuropathy and nephropathy and also of sequelae of diabetes mellitus in mammals.

The invention therefore relates also to a simple method of lowering the blood sugar concentration in mammals with the aid of dipeptide compounds as activity-reducing effectors (substrates, pseudosubstrates, inhibitors, binding proteins, antibodies etc.) for enzymes having activity comparable to or identical to the enzymatic activity of the enzyme dipeptidyl peptidase IV.

DP IV or DP IV-analogous activity (for example the cytosolic DP II has a substrate specificity almost identical to DP IV) occurs in the blood circulation where it splits off dipeptides highly specifically from the N-terminus of biologically active peptides when proline or alanine are the adjacent residues of the N-terminal amino acid in their sequence.

The glucose-dependent insulinotropic polypeptides: gastric inhibitory polypeptide 1-2 (GIP₁₋₄₂) and glucagonlike peptide amide-1 7-36 (GLP-1₇₋₃₆), that is to say hormones that stimulate glucose-induced secretion of insulin by the pancreas (also called incretins), are substrates of DP IV, since the latter is able to split off the dipeptides tyrosinyl-alanine and histidylalanine, respectively, from the N-terminal sequences of those peptides in vitro and in vivo.

The reduction of such DP IV and DP IV-analogous enzyme activity of the cleavage of those substrates in vivo can be used to bring about effective suppression of undesired enzyme activity under laboratory conditions and also in the case of pathological conditions in mammalian organisms. For example, diabetes mellitus Type II (including adult-onset diabetes) is based on a reduced secretion of insulin or disorders in the receptor function resulting inter alia from anomalous incretin concentrations arising from proteolysis.

The aim of the invention is therefore to provide new (especially activity-reducing) effectors for the treatment of e.g. impaired glucose tolerance, glycosuria, hyperlipidaemia, metabolic acidoses, diabetes mellitus, diabetic neuropathy and nephropathy and also of sequelae of diabetes mellitus in mammals, and a simple method of treating such diseases.

This aim is achieved according to the invention by the provision of dipeptide compounds or analogues of dipeptides that are formed from an amino acid and a thiazolidine or pyrrolidine group, and salts thereof.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts capillary zone electrophoretic separation of the isomers of isoleucyl thiazolide this separation is representative of a 1:1:1:1 mixture of L-threo-Ile-Thi*fum, L-allo-Ile-Thia*fum, D-threo-Ile-Thia*fum, D-allo-Ile-Thia*fum;

FIG. 2 depicts capillary zone electrophoretic separation of Ile-Thia*fumarate this separation is representative of a 1:1000 mixture of L-threo-Ile-Thia*fumarate to D-allo-Ile-Thia*fumarate;

FIG. 3 depicts a graphic representation of serum DP VI activity after oral administration of various H-Ile-Thia stereoisomers (5 μM/300 g rat). Enazyme activity influenced only by L-Allo-Ile-Thia and L-threo-Ile-Thia; and

FIG. 4 depicts action of various aminoacyl-thiazolidides on the glucose tolerance of rats (oral glucose tolerance test with 2 g/300 g Wistar rat at time point, administration of DP IV inhibitors 10 minutes prior to oral glucose stimulation).

DETAILED DESCRIPTION OF THE INVENTION

On administration, preferably oral administration, of these effectors to a mammalian organism, the endogenous (or additionally exogenously administered) insulinotropic peptides GIP₁₋₄₂ and GLP-1₇₋₃₆ (or alternatively GLP-1₇₋₃₇ or analogues thereof) are broken down to a reduced extent by DP IV or DP IV-like enzymes and therefore the decrease in the concentration of those peptide hormones or their analogues is reduced or delayed. The invention is therefore based on the finding that a reduction in the DP IV or DP IV-like enzymatic activity acting in the blood circulation has an effect on the blood sugar level. It has been found that

-   1. the reduction in DP IV or DP IV-analogous activity leads to an     increase in the relative stability of the glucose-stimulated or     externally introduced incretins (or analogues thereof), that is to     say by administration of effectors of DP IV or DP IV-analogous     proteins it is possible to control the breakdown of incretin in the     blood; -   2. the increase in the biological breakdown stability of the     incretins (or their analogues) results in a change in the action of     endogenous insulin; -   3. the increase in the stability of the incretins brought about by     the reduction in DP IV or DP IV-analogous enzymatic activity in the     blood results in a subsequent change in the glucose-induced insulin     action and therefore in a modulation of the blood glucose level that     is controllable by means of DP IV-effectors.

Especially suitable for that purpose according to the invention are dipeptide compounds in which the amino acid is selected from a natural amino acid, such as, for example, leucine, valine, glutamine, proline, isoleucine, asparagine and aspartic acid.

The administration, where possible oral administration, of the high-affinity, low molecular weight enzyme inhibitors according to the invention is a more economical alternative e.g. to invasive surgical techniques in the treatment of pathological symptoms. Through a chemical design of stability, transport and clearance properties, their mode of action can be modified and matched to individual characteristics.

As mentioned above, it may be necessary, for example in the case of the long-term treatment of diabetes mellitus, to provide effectors having a defined activity with which it is possible to meet the individual needs of patients and to treat their symptoms. The dipeptide compounds according to the invention therefore exhibit at a concentration (of dipeptide compounds) of 10 μM, especially under the conditions indicated in Table 1, a reduction in the activity of dipeptidyl peptidase IV or DP IV-analogous enzyme activities of at least 10%, especially of at least 40%. Frequently a reduction in activity of at least 60% or at least 70% is also required. Preferred effectors may also exhibit a reduction in activity of a maximum of 20% or 30%. Furthermore, the transport properties of the present compounds, especially by the peptide transporter Pep T1, are significantly improved.

Especially preferred dipeptide compounds are L-allo-isoleucyl thiazolidide and salts thereof. Those compounds surprisingly exhibit an approximately five-fold improvement in transport by the peptide transporter Pep T1 in comparison with L-threo-isoleucyl thiazolidide, while having approximately the same degree of action with respect to glucose modulation.

Further illustrative compounds are given in Table 1.

The salts of the dipeptide compounds according to the invention may be, for example, organic salts such as acetates, succinates, tartrates or fumarates, or inorganic acid radicals such as phosphates or sulphates. Special preference is given to the fumarates, which have an excellent action combined with a surprisingly high degree of stability towards hydrolysis and are considerably less soluble than the hydrochlorides. Those properties are also advantageous from the galenical standpoint.

Also preferred are L-threo-isoleucyl pyrrolidide and salts thereof, especially the fumaric salts, and L-allo-isoleucyl pyrrolidide and salts thereof, especially the fumaric salts.

The salts of the dipeptide compounds can be present in a molar ratio of dipeptide (-analogous) component to salt component of 1:1 or 2:1. Such a salt is, for example, (Ile-Thia)₂ fumaric acid.

Especially preferred salts are the fumaric salts of L-threo-isoleucyl thiazolidide and L-allo-isoleucyl thiazolidide.

The invention accordingly relates to effectors of dipeptidyl peptidase IV (DP IV) or DP IV-analogous enzyme activity and their use in lowering the blood sugar level in the serum of a mammalian organism below the glucose concentration that is characteristic of hyperglycaemia. The invention relates especially to the use of the effectors of DP IV or DP IV-analogous enzyme activity according to the invention in preventing or alleviating pathological metabolic anomalies in mammalian organisms, such as, for example, impaired glucose tolerance, glycosuria, hyperlipidaemia, metabolic acidoses, diabetes mellitus, diabetic neuropathy and nephropathy and also sequelae or diabetes mellitus in mammals. In a further embodiment, the invention relates to a method of lowering the blood sugar level in the serum of a mammalian organism below the glucose concentration that is characteristic of hyperglycaemia, characterised in that a therapeutically effective amount of at least one effector of DP IV or DP IV-analogous enzyme activity according to the invention is administered to a mammalian organism.

In an alternative illustrative embodiment, the invention relates to pharmaceutical compositions, that is to say medicaments, that comprise at least one compounds according to the invention or a salt thereof, optionally in combination with one or more pharmaceutically acceptable carriers and/or solvents.

The pharmaceutical compositions may be, for example, in the form of parenteral or enteral formulations and may contain appropriate carriers or they may be in the form of oral formulations that may contain appropriate carriers suitable for oral administration. They are preferably in the form of oral formulations.

In addition, the pharmaceutical compositions may contain one or more active ingredients having a hypoglycaemic action, which may be active ingredients known by those skilled in the art.

The effectors of DP IV or DP IV-analogous enzyme activity according to the invention can be used for lowering the blood sugar level in the serum of a mammalian organism below the glucose concentration that is characteristic of hyperglycaemia or for the production of a corresponding medicament.

The effectors of DP IV or DP IV-analogous enzymes administered according to the invention can be used in pharmaceutically acceptable formulations or formulation complexes as inhibitors, substrates, pseudosubstrates, inhibitors of DP IV expression, binding proteins or antibodies of those enzyme proteins or combinations of those different substances that reduce the DP IV or DP IV-analogous protein concentration in the mammalian organism. Effectors according to the invention are, for example, DP IV-inhibitors such as the dipeptide derivatives or dipeptide mimetics L-allo-isoleucyl thiazolidide and the effectors indicated in Table 1 and fumaric salts thereof. The effectors according to the invention enable the treatment of patients and diseases to be adjusted individually, it being possible especially to avoid intolerances, allergies and side effects occurring in individual cases.

The compounds also exhibit different effectiveness behaviours over time. As a result, the physician carrying out the treatment has the opportunity to respond in various ways according to the individual situation of a patient: he is able, on the one hand, to set accurately the speed of onset of the action and, on the other hand, the duration of action and especially the strength of action.

The method according to the invention represents a new kind of procedure for lowering raised blood glucose concentrations in the serum of mammals. It is simple, capable of commercial exploitation and suitable for use in therapy, especially of diseases that are based on above-average blood glucose values, in mammals and more especially in human medicine.

The effectors are administered, for example, in the form of pharmaceutical preparations that comprise the active ingredient in combination with customary carrier materials known in the prior art. For example, they will be administered parenterally (e.g. i.v., in physiological saline) or enterally (e.g. orally, formulated with customary carrier materials, such as, for example, glucose).

Depending upon their endogenous stability and their bioavailability, the effectors will need to be administered one or more times per day in order to achieve the desired normalisation of the blood glucose values. For example, such a dosage range in human beings may lie in the range of from 0.01 mg to 30.0 mg per day, preferably in the range of from 0.01 to 10 mg of effector substance per kilogram of body weight.

It has been found that as a direct result of the administration of effectors of dipeptidyl peptidase IV or DP IV-analogous enzyme activities in the blood of a mammal, by virtue of the associated temporary reduction in the activity thereof, the endogenous (or additionally exogenously administered) insulinotropic peptides gastric inhibitory polypeptide 1-42 (GIP₁₋₄₂) and glucagon-like peptide amide-1 7-36 (GLP-1₇₋₃₆) (or alternatively GLP-1₇₋₃₇) or analogues thereof) are broken down to a reduced extent by DP IV and DP IV-like enzymes and thus the decrease in the concentration of those peptide hormones or their analogues is reduced or delayed. The increase in the stability of the (endogenously present or exogenously introduced) incretins or their analogues brought about by the action of DP IV-effectors, with the result that the former are available in increased amounts for insulinotropic stimulation of the incretin receptors of the Langerhan's cells in the pancreas, alters inter alia the effectiveness of the body's own insulin, which results in a stimulation of the carbohydrate metabolism of the treated organism.

As a result, the blood sugar level in the serum of the organism being treated falls below the glucose concentration that is characteristic of hyperglycaemia, thus making it possible to prevent or alleviate metabolic anomalies such as impaired glucose tolerance, glycosuria, hyperlipidaemia and possible severe metabolic acidoses and diabetes mellitus, which are clinical syndromes resulting from raised glucose concentrations in the blood over a prolonged period.

Among the number of orally effective anti-diabetics known from the prior art, such an effective low molecular weight substance class has been unknown hitherto (with the exception of the biguanide metformin: molecular weight 130). The molecular weights of the aminoacyl thiazolidides vary between 146 (glycyl thiazolidide), 203 (isoleucyl thiazolidide) and 275 (tryptophanyl thiazolidide). In comparison, the molecular weights of the sulphonylureas (glibenclamide: 494), the saccharides (acarbose: 630) and the thiazolidinediones (pioglitazon: 586) vary in the range around 500 to 700 Da. In the body, aminoacyl thiazolidides are hydrolysed by aminopeptidases and by acidic hydrolysis to form endogenous substances, such as amino acids and cysteamine, so that the use of the compounds according to the invention as orally available anti-diabetics constitutes an enrichment of pharmacy.

In rats and mice, experimentally induced hyperglycaemia can be treated to a better than average extent by oral administration of the compounds used according to the invention as shown in Tables 2 and 3. The administration of 500 to 1000 times the effective dose did not result in any demonstrable pathological change during three-week toxicological experiments on rats and mice.

The advantageous action of compounds according to the invention on DP IV is shown by way of example in Table 1: TABLE 1 Action of various effectors on the dipeptidyl-peptidase-IV-catalysed hydrolysis of 0.4 mM of the substrate H-Gly-Pro-pNA at 30° C., pH 7.6 and an ionic strength of 0.125 Effector affinity % Residual activity of to DP IV: DP IV in the presence of Effector K_(i) [nM] 10 μM effector metformin >>1,000,000 100 glibenclamide >>1,000,000 100 acarbose >>1,000,000 100 H-Asn-pyrrolidide 12,000 83.1 H-Asn-thiazolidide 3,500 47.2 H-Asp-pyrrolidide 14,000 81.6 H-Asp-thiazolidide 2,900 45.6 H-Asp(NHOH)-pyrrolidide 13,000 88.2 H-Asp(NHOH)-thiazolidide 8,800 54.5 H-Glu-pyrrolidide 2,200 38.5 H-Glu-thiazolidide 610 25.0 H-Glu(NHOH)-pyrrolidide 2,800 44.9 H-Glu(NHOH)-thiazolidide 1,700 36.5 H-His-pyrrolidide 3,500 49.7 H-His-thiazolidide 1,800 35.2 H-Pro-pyrrolidide 4,100 50.2 H-Pro-thiazolidide 1,200 27.2 H-Ile-azididide 3,100 43.8 H-Ile-pyrrolidide 210 12.3 H-L-allo-Ile-thiazolidide 190 10.0 H-Val-pyrrolidide 480 23.3 H-Val-thiazolidide 270 13.6

It is known that aminoacyl pyrrolidides and aminoacyl thiazolidides can be broken down by the enzymes proline aminopeptidase and prolidase present in the mucosa cells of the small intestine, in serum and in liver cells and that the thiazolidine ring has a tendency to open in the presence of acids (for example in the stomach) with the formation of the corresponding cysteamine derivative. It was therefore surprising to find that the active ingredients have a dose-dependent effectiveness after peroral administration. The dose-dependency of the action of L-allo-Ile-thiazolidide on the serum-DP IV activity after oral administration of L-allo-isoleucyl thiazolidide to healthy Wistar rats is documented in the following Table: TABLE 2 Residual activity of DP IV in serum towards 0.4 mM of the substrate H-Gly-Pro-pNA at 30° C., pH 7.6 and an ionic strength of 0.125, after oral administration and in dependence upon the dose of L-allo-isoleucyl thiazolidide, determined 30 min after administration of the inhibitor Dose per experimental animal Residual activity of DP IV in %   0 mg 100 2.5 mg 52 5.0 mg 40  10 mg 28  20 mg 29

Extremely surprising and desirable is the glucose-reducing action of the active ingredient L-allo-isoleucyl thiazolidide according to the invention achieved in the diabetic animal model after oral administration with synchronous oral glucose stimulation as shown in Table 3.

In order to intensify the blood-sugar-reducing action of various anti-diabetics, use is frequently made of combinations of different orally effective anti-diabetics. Since the anti-hyperglycaemic action of the effectors according to the invention is exhibited independently of other known oral anti-diabetics, the active ingredients according to the invention are analogously suitable for use in combination therapies, in a suitable galenical form, for achieving the desired normoglycaemic effect.

Accordingly, the compounds used according to the invention can be made in a manner known by those skilled in the art into the customary formulations, such as, for example, tablets, capsules, dragees, pills, suppositories, granules, aerosols, syrups, liquid, solid and cream-type emulsions and suspensions and solutions using inert, non-toxic, pharmaceutically acceptable carriers and additives or solvents. In such formulations the therapeutically effective compounds are in each case preferably present in a concentration of approximately from 0.1 to 80% by weight, preferably from 1 to 50% by weight, of the total mixture, that is to say in amounts sufficient to achieve a dosage within the indicated range. TABLE 3 Reduction in the circulating blood glucose within a period of 60 min after oral administration of 20 μM of L-allo-Ile thiazolidide to rats of various animal models with a synchronous glucose tolerance test (data in % based on normoglycaemic values). Glucose Glucose concentration concentration in % in % Animal model control L-allo-Ile-thiazolidide-treated Wistar rat, normal 100 82 Wistar rat 100 73 (diabetes 2b- model, obese)

The good absorption of the compounds used according to the invention by mucosae of the gastro-intestinal tract enables a large number of galenical preparations to be used:

The substances can be administered as medicaments in the form of dragees, capsules, bitable capsules, tablets, drops and syrup, as well as in the form of pessaries and nasal sprays.

The formulations are produced, for example, by extending the active ingredient with solvents and/or carriers, optionally using emulsifiers and/or dispersing agents, and optionally, for example where water is used as diluent, organic solvents may be used as auxiliary solvents.

The following auxiliaries may be mentioned by way of example: water, non-toxic organic solvents, such as paraffins (e.g. mineral oil fractions), vegetable oils (e.g. rapeseed oil, groundnut oil, sesame oil), alcohols (e.g. ethyl alcohol, glycerol), glycols (e.g. propylene glycol, polyethylene glycol); solid carriers, such as, for example, ground natural minerals (e.g. highly dispersed silicic acid, silicates), sugars (e.g. unrefined sugar, lactose and dextrose); emulsifiers, such as non-ionic and anionic emulsifiers (e.g. polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers, alkylsulphonates and arylsulphonates), dispersing agents (e.g. lignin, spent sulphite liquors, methylcellulose, starch and polyvinylpyrrolidone) and glidants (e.g. magnesium stearate, talcum, stearic acid and sodium lauryl sulphate) and optionally flavourings.

Administration is effected in customary manner, preferably enterally or parenterally, especially orally. In the case of enteral administration, in addition to containing the mentioned carriers, tablets may also comprise other additives, such as sodium citrate, calcium carbonate and calcium phosphate, together with various supplementary ingredients, such as starch, especially potato starch, gelatin and the like. It is also possible to use glidants, such as magnesium stearate, sodium lauryl sulphate and talcum, for tableting purposes. In the case of aqueous suspensions and/or elixirs intended for oral uses it is also possible for various taste correctors or colorings to be added to the active ingredients in addition to the auxiliaries mentioned above.

For parenteral administration it is possible to use solutions of the active ingredients using suitable liquid carrier materials. In the case of intravenous administration it has generally proved advantageous to administer amounts of approximately from 0.01 to 2.0 mg/kg, preferably approximately from 0.01 to 1.0 mg/kg, body weight per day in order to achieve effective results, and in the case of enteral administration the dosage is approximately from 0.01 to 2 mg/kg, preferably approximately from 0.01 to 1 mg/kg, body weight per day.

Nevertheless in some cases it may be necessary to depart from the amounts indicated, depending upon the body weight of the experimental animal or patient or the nature of the administration route, and also on the basis of the species of animal and its individual response to the medicament or the intervals at which the administration is made. In some cases, for example, it may be sufficient to use less than the above-mentioned minimum amount, whereas in other cases it will be necessary to exceed the above-mentioned upper limit. Where relatively large amounts are administered it may be advisable to divide the amount into several individual doses over the day. For use in human medicine the same range of dosage is provided, the comments made above also applying accordingly.

Examples of Pharmaceutical Formulations

-   1. Capsules having 100 mg of L-allo-isoleucyl thiazolidide per     capsule:

For about 10,000 capsules, a solution of the following composition is prepared: L-allo-isoleucyl thiazolidide hydrochloride 1.0 kg glycerol 0.5 kg polyethylene glycol 3.0 kg water 0.5 kg 5.0 kg

The solution is introduced into soft gelatin capsules in a manner known by those skilled in the art. The capsules are suitable for chewing or swallowing.

-   2. Tablets/coated tablets or dragees having 100 mg of     L-allo-isoleucyl thiazolidide:

The following amounts relate to the production of 100,000 tablets: L-allo-isoleucyl thiazolidide hydrochloride, 10.0 kg finely ground glucose 4.35 kg lactose 4.35 kg starch 4.50 kg cellulose, finely ground 4.50 kg

The above constituents are mixed together and then combined with a solution, prepared from polyvinylpyrrolidone 2.0 kg polysorbate 0.1 kg and water about 5.0 kg and granulated in a manner known by those skilled in the art by grating the moist mass and, after the addition of 0.2 kg of magnesium stearate, drying. The finished tablet mixture of 30.0 kg is processed to form domed tablets each weighing 300 mg. The tablets can be coated or sugar-coated in a manner known by those skilled in the art.

The technical data of illustrative compounds are shown in Table 4. TABLE 4 Tests on Ile-Thia*fumarate (isomer) and other salts Substance K_(i) Mp (° C.) CE (min) MS [α]H₂0 L-threo-IT*F 8*10⁻⁸ 150^(DSC) 160 203 −10.7 (405 nm) D-threo-IT*F no 147 158 203 not inhibition determined L-allo-IT*F 2 * 10⁻⁷ 145-6 154 203 −4.58 (380 nm) D-allo-IT*F no 144-6 150 203 4.5 inhibition (380 nm) IT*F = isoleucyl thiazolidide fumarate

The NMR and HPLC data confirm the identity of the substances in question.

Measurement Conditions for the K_(i) Determination of the Substances

-   Enzyme: DP Iv_(porcine kidney), 0.75 mg/ml, 18 U/ml (GPpNA) in 25 mM     Tris pH 7.6, 30% ammonium sulphate, 0.5 mM EDTA, 05 mM DTE     -   Stock solution: 1:250 diluted in measuring buffer -   Buffer: 40 mM HEPES pH 7.6, I=0.125 (KCl) -   Substrate: GPpNA*HCl     -   Stock solution: 2.1 mM -   Measuring -   apparatus: Perkin-Elmer Bio Assay Reader, HTS 7000 Plus,     -   T=30° C.     -   λ=405 nm -   Measurement -   batch: 100 μl buffer     -   100 μl substrate (3 different concentrations 0.8 mM-0.2 mM)     -   50 μl water/inhibitor (7 different concentrations 2.1 μM-32.8         nM)     -   10 μl enzyme

Buffer, water/inhibitor and enzyme were preheated to 30° C. and the reaction was started by the addition of substrate which was likewise preheated. Determinations were carried out four times. The measuring time was 10 minutes.

Melting Point Determination

Melting points were determined on a Kofler heating platform microscope from Leica Aktiengesellschaft, the values are not corrected, or on a DSC apparatus (Heumann-Pharma).

Optical Rotation

The rotation values were recorded at different wavelengths on a “Polarimeter 341” or higher, from the Perkin-Elmer company.

Measurement Conditions for the Mass Spectroscopy

The mass spectra were recorded by means of electrospray ionisation (ESI) on an “API 165” or “API 365” from the PE Sciex Company. The operation is carried out using an appropriate concentration of c=10 μg/ml, the substance is taken up in MeOH/H₂O 50:50, 0.1% HCO₂H, the infusion is effected using a spray pump (20 μl/min). The measurements were made in positive mode [M+H]⁺, the ESI voltage is U=5600V.

The salts have the following data which is shown in Table 5.: TABLE 5 IT*salt K_(i) M (gmol-¹) Mp (° C.) succinate 5.1e−8 522.73 116 tartrate 8.3e−8 352.41 122 fumarate 8.3e−8 520.71 156 hydrochloride 7.2e−8 238.77 169 phosphate 1.3e−7 300.32 105 Testing the Solubility of Salts of Ile-Thia Ile-Thia*fum

-   Amount weighed in 10.55 mg -   corresponds to 0.02 mmol (520.72 g/mol) -   Addition of 100 μl H₂O_(dist). -   100 μl no solution, visually: no surface-wetting -   from 200 μl successive beginning of solubility -   at 400 μl complete dissolution is observed -   2.63%

It is therefore established that this salt is scarcely wettable and does not decompose.

Ile-Thia*succ

-   Amount weighed in 16.6 mg -   corresponds to 0.031 mmol (522.73 g/mol) -   Addition of 16 μl H₂O_(dist). -   16 μl no solution, visually: “sucking-up” of the moisture from 66     μl-1.5 ml no complete dissolution of the substance is observed     Ile-Thia*tartrate -   Amount weighed in 17.3 mg -   corresponds to 0.049 mmol (352.41 g/mol) -   Addition of 100 μl H₂O_(dist). -   100 μl complete dissolution -   17.3%     Ile-Thia*phos -   Amount weighed in 15.5 mg -   corresponds to 0.051 mmol (300.32 g/mol) -   Addition of 100 μl H₂O_(dist). -   100 μl slight dissolution is observed -   successive addition of 100 μl H₂O -   at 400 μl complete dissolution -   3.87%     Ile-Thia*HCl -   Amount weighed in 16.1 mg -   corresponds to 0.067 mmol 238.77 (g/mol) -   Addition of 100 μl H₂O_(dist). -   at 100 μl complete dissolution -   16.1%     General Synthesis of Ile-Thia*Salt

The Boc-protected amino acid Boc-Ile-OH is placed in ethyl acetate and the batch is cooled to about −5° C. N-Methylmorpholine is added dropwise, pivalic acid chloride (on a laboratory scale) or neohexanoyl chloride (on a pilot-plant scale) is added dropwise at constant temperature. The reaction is stirred for a few minutes for activation. N-Methylmorpholine (laboratory scale) an thiazolidine hydrochloride (laboratory scale) are added dropwise in succession, thiazolidine (pilot-plant scale) is added. Working-up in the laboratory is effected in conventional manner using salt solutions, on a pilot-plant scale the batch is purified with NaOH and CH₃COOH solutions. The removal of the Boc protecting group is carried out using HCl/dioxane (laboratory scale) or H₂SO₄ (pilot-plant scale). In the laboratory the hydrochloride is crystallised from EtOH/ether. On a pilot-plant scale the free amine is prepared by the addition of NaOH/NH₃. Fumaric acid is dissolved in hot ethanol, the free amine is added dropwise, and (Ile-Thia)₂ fumarate (M=520.71 gmol-¹) precipitates.

The analysis of isomers and enantiomers is carried out by electrophoresis. 

1-18. (canceled)
 19. A pharmaceutical composition, comprising a pharmaceutically acceptable amount of an effector of dipeptidyl peptidase IV or dipeptidyl peptidase IV analogous enzyme activity in combination with a further anti-diabetic agent, or pharmaceutically acceptable salts thereof.
 20. The pharmaceutical composition according to claim 19 wherein said effector is selected from the group consisting of substrates, pseudosubstrates, inhibitors, binding proteins and antibodies.
 21. The pharmaceutical composition according to claim 19 wherein said effector is a dipeptide mimetic inhibitor comprising an amino acid and a pyrrolidine or thiazolidine group.
 22. The pharmaceutical composition according to claim 21 wherein said dipeptide mimetic inhibitor is selected from the group consisting of L-threo-isoleucyl pyrrolidine, L-threo-isoleucyl thiazolidine, L-allo-isoleucyl thiazolidine, L-allo-isoleucyl pyrrolidine, glutaminyl pyrrolidine, glutaminyl thiazolidine, alanyl thiazolidine, alanyl pyrrolidine, valyl thiazolidine and valyl pyrrolidine.
 23. The pharmaceutical composition according to claim 22 wherein said further anti-diabetic agent is selected from the group consisting of biguanides, sulphonylureas, saccharides and thiazolidinediones.
 24. The pharmaceutical composition according to claim 23 wherein said biguanide is metformin.
 25. The pharmaceutical composition according to claim 23 wherein said sulphonylurea is glibenclamide.
 26. The pharmaceutical composition according to claim 23 wherein said saccharide is acarbose.
 27. The pharmaceutical composition according to claim 23 wherein said thiazolidinedione is pioglitazon.
 28. The pharmaceutical composition according to claim 19 further comprising a pharmaceutically acceptable carrier.
 29. A process for preparing a pharmaceutical composition comprising an effector of dipeptidyl peptidase IV or dipeptidyl peptidase IV analogous enzyme activity, a further anti-diabetic agent or pharmaceutically acceptable salts thereof, and a pharmaceutically acceptable carrier therefore, which process comprises admixing the dipeptidyl peptidase IV effector, the further anti-diabetic agent, or pharmaceutically acceptable salts thereof, and a pharmaceutically acceptable carrier.
 30. A method for the treatment of metabolic disorders comprising administering to a mammal a therapeutically effective amount of the pharmaceutical composition of claim
 19. 31. The method according to claim 30 wherein said effector is a dipeptide mimetic inhibitor comprising an amino acid and a pyrrolidine or a thiazolidine group, or pharmaceutically acceptable salts thereof.
 32. The method according to claim 31 wherein said dipeptide mimetic inhibitor is selected from the group consisting of L-threo-isoleucyl pyrrolidine, L-threo-isoleucyl thiazolidine, L-allo-isoleucyl thiazolidine, L-allo-isoleucyl pyrrolidine, glutaminyl pyrrolidine and glutaminyl thiazolidine, alanyl thiazolidine, alanyl pyrrolidine, valyl thiazolidine and valyl pyrrolidine, and pharmaceutically acceptable salts thereof.
 33. The method according to claim 32 wherein said further anti-diabetic agent is selected from the group consisting of biguanides, sulphonylureas, saccharides and thiazolidinediones.
 34. The method according to claim 33 wherein said biguanide is metformin.
 35. The method according to claim 33 wherein said sulphonylurea is glibenclamide.
 36. The method according to claim 33 wherein said saccharide is acarbose.
 37. The method according to claim 33 wherein said thiazolidinedione is pioglitazon.
 38. The method according to claim 30 wherein said metabolic disorder is selected from the group consisting of impaired glucose tolerance, glucosuria, hyperlipidaemia, metabolic acidosis, diabetic neuropathy, nephropathy, diabetes mellitus and sequelae of diabetes mellitus.
 39. The method according to claim 30 wherein said administering said pharmaceutical composition results in an increase in stability of at least one of endogenously present or exogenously introduced GIP₁₋₄₂, GLP-1₇₋₃₆ and GLP-1₇₋₃₇ or analogues thereof.
 40. The method according to claim 39 wherein said treatment method causes an increase in incretin levels for stimulating incretin receptors on Langerhan's cells.
 41. The method according to claim 39 wherein said treatment method results in increased effectiveness of the Langerhan's cells.
 42. The method according to claim 39 wherein said treatment method results in increased stimulation of carbohydrate metabolism of the treated mammal.
 43. The method according to claim 40 wherein said treatment method results in lowering the blood glucose level in the serum of the mammal being treated below the glucose concentration that is characteristic of hyperglycaemia in said mammal.
 44. The method according to claim 30, wherein said pharmaceutical composition is administered orally. 